Rapid multiplication of Zingiber cassumunar using axillary bud explant

An in vitro regeneration system was standardized on a traditional medicinal plant of Zingiber cassumunar. The rhizome axillary buds upon transfer to MS basal medium with benzyl adenine (3mg/l) found optimum exhibiting maximum percent of shoot initiation as 92.5 ± 0.6, thereby maximum number of shoots as 6.8 ± 0.3 and maximum number of roots as 22.0 ± 0.4 were found in benzyl adenine (3mg/l) and indole acetic acid (0.5mg/l) combination. The micropropagated plants were transferred to field for successful establishment as 90% respectively. Furthermore, the morphological characteristic analysis was done between the conventional and micropropagated plants which showed very less significant variation. The present protocol reports the propagation of Z. cassumunar plantlets which could be used for commercial exploitation in future.