The major fatty acids present in cosmetics are the unsaturated fatty acids from triglycerides, especially essential fatty acids: linoleic acid (omega-6) and α-linolenic acid (omega-3).The purpose of the study was to develop a simple and precise gas chromatography-flame ionization detection method, using an OPTIMA-WAX (macrogol 20000) capillary GC column (30m x 0.32mm x 0.25µm) with a run time of 17min, for the analysis of fatty acids composition from vegetable oils and macerated oils.The method was validated for quantifying four major fatty acids: palmitic, stearic, oleic and linoleic acids, as methyl esters.The quantification was performed by internal standardization, using the methyl ester of nonadecanoic acid as internal standard.The esterification reaction was carried out on a magnetic stirrer at a temperature of 80°C and with continuous stirring, in hermetically sealed vials.
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Fiastru-Irimescu et al. (2024) studied this question.
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