FIGURE 7 from MARCH2, a Novel Oncogene-regulated SNAIL E3 Ligase, Suppresses Triple-negative Breast Cancer Metastases

MARCH2 suppresses metastases of TNBC cells. A, Metastatic lung colonization by MDA-MB-231/Luciferase cells expressing vector control, WT MARCH2, or RING domain–mutant (W97A) MARCH2 following tail vein injection was serially assessed by IVIS imaging. Total photons/second in a 3.3 × 3.3 cm² area around the lung area of each mouse was used for statistical analysis. P values were calculated by Student t test, and the P value less than 0.05 was regarded as statistically significant (*, P < 0.05; **, P < 0.01; ***, P < 0.001). Data represent mean ± SD. B, Spontaneous metastases formation in the lungs by MDA-MB-231/LM2-4/Luciferase cells expressing vector control or WT MARCH2 following implantation into left fourth mammary fat pad implantation was serially assessed by IVIS imaging. Total photons/second in a 3.3 × 3.3 cm² area around the upper body area of each mouse was used for statistical analysis. P values were calculated by Student t test. Data represent mean ± SD.