Effect of Plant Growth hormones through in vitro technique on different types of seaweeds like Sargassum tenerrimum and Gracilaria corticata collected from Indian coastal areas

Plant tissue culture means culturing plant seeds, organs, explants, tissues, cells, or protoplasts on a chemically defined synthetic nutrient media under sterile and controlled conditions of light, temperature, and humidity.Tissue culture techniques play a significant role in geophysiological process, strain improvement, morphogenesis and micropropagation for any types of crops.In this technique, the influence of different concentration of plant growth hormones such as auxin, cytokinin, gibberellin and IBA enriched marine media were used.Amongst different concentration of hormones enriched media, auxin 10 -6 M enriched PES and f/2 media influences maximum number of vegetative branches and a callus (disorganized cell mass that arose from the organized tissue of the explant), Although regeneration was observed in other supplemented cytokinin, gibberellin and IBA enriched media but the speed of regeneration and growth was found to be slow compared to auxin.The simultaneous formation of filamentous outgrowth was observed at the cut ends of explants.These filamentous outgrowths later developed several vegetative branches cultured in different concentration of enriched media.The explants taken from the basal portion of the thallus regenerated maximum number of vegetative branches compared to the apical portion.In G. corticata, the pattern of the growth of vegetative Effect of Plant …