LipidOne 2.0: a web tool for discovering biological meanings hidden in lipidomic data

Two years after the release of LipidOne's desktop version, we introduce the updated web platform version: LipidOne 2.0 (https://lipidone.eu).This bioinformatics tool introduces several innovations in the analysis of lipidomic data, facilitating the exploration of the three structural levels of lipids: classes, molecular species and lipid building blocks (acyl, alkyl or alkenes chains).The tool's flexibility empowers users to seamlessly include or exclude experimental groups and lipid classes at any stage of the analysis.The updated version offers a range of mono-and multivariate statistical analyses, specifically tailored to each structural level.This includes a novel lipid biomarker identification function, integrating four diverse statistical parameters.A noteworthy improvement in LipidOne 2.0 is the incorporation of Lipid Pathway analysis across all three structural levels of lipids.Users can identify lipid-involved reactions through case-control comparisons, generating lists of genes/enzymes and their activation states based on Z scores.Accessible without the need for registration, LipidOne 2.0 provides a user-friendly and efficient platform for exploring and analyzing lipidomic data.