A Novel Approach for Evaluation of Hesperidin in Vincristrine-Induced Neuropathy in Rats

The present study set out to determine how hesperidin altered the neuropathic pain that vincristine-induced on rats. Rats were given vincristine to cause painful neuropathy. To measure the mechanical dynamic allodynia, cold allodynia, degree of mechanical hyperalgesia, heat hyperalgesia in addition to muscle relaxants rota rod, respectively, several pain-sensitive tests, including the von frey hair test pinprick, hot plate, and rota rod, were carried out on various weeks (00, 04 and 08 week ). As indicators of inflammation and oxidative stress, the IL-1β, IL-10, and tumor necrosis factor-α, tissue parameters like Na+/K+ ATPase, Ca2+ ATPase & Mg2+ ATPase, and SOD, CAT level, reduced glutathione (GSH), lipid peroxidase (LPO), NO level were assessed. Gabapentin (30 mg/kg i.p.) in addition to hesperidin (50, 100, as well as 150 mg/kg orally) were given for 08 weeks. Hesperidin administration significantly reduced vincristine-induced behavioral and biochemical alterations (p <0.05). Hesperidin also reduced the inflammation IL-10, and increased in IL-1ß, and TNF-ɑ. Hesperidin also reduces oxidative stress (LPO, NO level) and increases at (GSH, SOD, CAT levels) that vincristine caused. Hesperidin can alleviate the painful states brought on by vincristine-induced painful neuropathy, which might also be explained by its anti-inflammatory effects and following reduction of oxidative stress..