This protocol describes the steps taken to assess whether MR signal noise and distortion is introduced by utilization of a 3D printed plastic stereotaxic frame. The computer-aided design files and engineering drawings for the frame used in this protocol are publicly available, with the modular design allowing for low cost and manageable manufacturing. You can find it here: https://github.com/SchaefferLab/Macaque-Stereotax This protocol is supplementary to the manuscript: Liang, L., Zimmermann Rollin, I., Alikaya, A., Ho, J.C., Santini, T., Bostan, A.C., Schwerdt, H.N., Stauffer, W.R., Ibrahim, T.S., Pirondini, E., Schaeffer, D.J., 2024. An open-source MRI compatible frame for multimodal presurgical mapping in macaque and capuchin monkeys. BioRxiv https://doi.org/10.1101/2024.02.17.580767
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