Here we summarize the reccomendation released by 10X Genomics for processing fixed-frozen tissue sections while performing 10X Visium Spatial Gene Expression assay using the Visium CytAssist device. Please follow the link for the detailed official protocol on 10X Genomics website. https://cdn.10xgenomics.com/image/upload/v1680564483/support-documents/CG000662_Demonstrated_Protocol_VisiumCytAssist_FixedFrozen_H_E_RevA.pdf
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Pappalardo et al. (2024) studied this question.
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