Analytical Methods to Estimate the Amount of Additives in Confectioneries

The objective of the study is to develop simple, accurate and precise analytical method for the estimation of sucralose, which is used as an articial sweetener. The method was developed using UV-Visible spectrophotometer. UV-Visible spectrophotometric method using calibration curve, was established for the estimation. The developed method were validated according to ICH guidelines. The purity of drug was checked by using FTIR spectroscopy. Sucralose show better solubility in methanol. Sucralose is estimated using Romini's reagent which is a mixture of sodium nitroprusside, ZnCl2 and acetone. Romini's reagent in reaction with sucralose produces a compound which has max at 291.5nm. This method showed linearity within the range of 2-24µg/ml. Correlation coefcient was found to be 0.9995.The developed method were validated for linearity, accuracy, precision, intra-day and inter- day precision, limit of detection and limit of quantication. The method is simple, linear, precise, accurate and suitable for the estimation of sucralose. In conclusion, using these developed analytical method, analysis of the sucralose can be done accurately in a short time with low cost and without prior extraction.