Amperometric Biosensing of L-Glutamate Using Reduced Graphene Oxide and Glutamate Oxidase

The determination of L-glutamate in biological media is very important, as it is the most common excitatory neurotransmitter related to some neurological diseases, such as Parkinson's, communication dysfunction, stroke, epilepsy and schizophrenia. When aiming to study the pathways of these diseases, as well as for the evaluation of medical treatments, it is very important to have rapid and reliable methods for the determination of L-glutamate. This study presents the new approach of an enzyme-based biosensor operating at −0.1 V, which ensures its good sensitivity and selectivity. The reduced graphene oxide used in the biosensor allowed for the monitoring of L-glutamate via the electro-oxidation of the NH3 released during the reaction catalyzed by Glutamate oxidase.