Metabolic features of mitosis remain poorly understood because this phase of the cell cycle is rapid and heterogeneous between cells within a dish. Label-free optical metabolic imaging (OMI) can monitor rapid changes in cell metabolism with single cell resolution using two-photon microscopy of the optical redox ratio (NAD(P)H/FAD) and NAD(P)H fluorescence lifetimes. Here, we brought together image analysis tools to quantify OMI time-courses of single cells undergoing mitosis across multiple cell lines. Alignment of OMI and morphological features over time provided unique insight into metabolic changes during mitosis within unperturbed systems.
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Guzman et al. (2024) studied this question.
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