Two-photon microscopy with ultrafast scattering control for deep tissue imaging

Deep brain imaging at cellular resolution is challenging due to strong and dynamic tissue scattering. Previous techniques for scattering correction are either slow or not in reflection mode, hindering them from imaging deep in the mouse brain in vivo. Here, we have developed a new two-photon microscope with ultrafast scattering correction for deep brain imaging. We use a compressive sensing algorithm to calculate correction masks from much fewer measurements than previous methods and couple light into high-transmission channels of scattering tissue using a digital micromirror device. We demonstrate real-time correction of dynamic scattering by focusing through various dynamic scattering samples.