The neonatal mouse heart in contrast to the adults has the ability to regenerate after myocardial infarction. The investigation of cardiac morphogenesis is critical to uncover the underlying mechanism of cardiac regeneration. Hence, we have developed a light-sheet microscope (LSM) along with the tissue-clearing method to investigate the 3-dimensional (3D) architecture of the intact neonatal mouse heart. We improved our imaging system by incorporating an axially swept remote focusing arm with a voice coil actuator for isotropic imaging. Our LSM offers a lateral resolution of 2.08 ± 0.11 μm and an axial resolution of 2.84 ± 0.19 μm. Using this strategy, we can take optical sections throughout the intact heart and enabling us to reveal the cardiac structure of the neonatal mouse.
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Almasian et al. (2024) studied this question.
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