Development of Monoclonal Antibodies and Immunochromatographic Test Strips Applying Indirect Competitive Method for Daidzein Detection

In this study, we aimed to detect daidzein secreted in the soybean rhizosphere using an indirect competitive immunochromatographic assay.A specific anti-daidzein monoclonal antibody was obtained through the establishment of a hybridoma.The antibody exhibited a detection limit of 5 ppb daidzein and showed strong cross-reactivity with daidzein, as determined by an indirect competitive method using a surface plasmon resonance sensor.Through the findings, a unique binding pattern differing from those of genistein and other saponins was observed.Enhanced sensitivity with the ability to detect daidzein concentrations as low as 10 nM (2.5 ng/mL) was achieved using immunochromatographic test strips with modified gold nanoparticles.