Abstract 1393 Synthesis of Silver Nanoparticles with Agave americana Leaf extract used for Waste water treatment

Agave americana has been used by traditional herbalist to treat many diseases in African traditional medicine. There is a widespread anxiety about the scourge of diseases caused by contaminants in water, from waste channels in environment, which pose a danger to health of humans in the environment. Therefore, this research is aimed at evaluating the biological and characterization of the activity of Agave americana plant extracts. The powdered Leaves was evaluated for its biological profiles and then extracted successively by maceration method using water. The extracts obtained was concentrated using rotary evaporator with 10% percentage yields of the extracts were determined. Quantitative phytochemical constituents of standard protocols were done, which reveal the presence of flavonoids, phenols, tannins, saponins and alkaloids. Elemental analysis of Synthesized Nanoparticles (AgNp) was carried out using Scanning Electron Machne, X-ray Diffractor and Zeta size analyzer. The plant's identity has been established to be Agave americana from physichochemical evaluation carried out. The antibacterial activity demonstrated by the extract was concentration dependent with showing susceptibility at 20mg/mL. The tested multidrug-resistant strains of bacteria were susceptible to the potential antibacterial action of the produced AgNPs. The AgNPs size was 90nm in size, the SEM showed the particles were spherical and stable, the XRD results revealed the picks correlated to silver nitrate. In order to clean waste water in the community and stop the spread of water related diseases, these AgNPs could potentially be a source for the creation of extremely effective antimicrobial coatings.