Effect of Polymorphisms of Vascular Endothelial Growth Factor (VEGF) and Transforming Growth Factor Beta-1 (TGF-β1) in Women with Recurrent Miscarriage

AbstractThe current study was conducted in the laboratories of Thi-Qar University's Research and marshes center in collaboration with (Bint Al Huda Hospital) from February 2023 to August 2023. The aim of the current study was to investigating the allelic association of a single nucleotide polymorphism in VEGF and TGFβ1genes with Recurrent miscarriage incidence by using PCR and gel electrophoresis. The study included 100 human blood samples with recurrent miscarriage women and 50 human blood samples as control group their ages ranged were between 17- 45 years. The patients and control were divided into three groups according to their age stages. The first group aged 17-26 years, the second group aged 27 – 36 years, and the third group aged37- 46 years. The VEGF and TGFβ1 promoter genes polymorphism (182 G/C) (488 C/T) promoter gene polymorphism was identified, and genotyping was verified by direct sequencing. The results of the statistical analysis showed no correlation between the occurrence of recurrent miscarriage and the emergence of mutation VEGF and TGFβ1 genes at p. value < 0.05. when it was compared with the control in some population of Thi-Qar province. Conclusions This research confirms that polymorphisms in the VEGF and TGFB1 genes have no impact on the prevalence of RM in Thi-Qar communities.