Anti-oxidant and cytotoxic effect of collagen extracted from local buffalo

This study about aimed to consider the DPPH radical scavenging recreation and cytotoxicity potentials of collagen extracted from buffalo. The result of antioxidant (DPPH) indicated that collagen possessed the ability to scavenge free in a concentrations dependant manner. They were (67.86±3.65, 54.44± 2.05, 47.92 ±0.53, 44.29±1.80 and 18.02±5.74%) for (200,100,50,25 and 12.5 mg) respectively. the results of cytotoxicity assay match the antioxidant results, the cell viability decrease by concentrations increasing to be 40.20± 3.06, 48.64± 1.48, 61.57± 1.33, 71.14± 0.82 and 83.21± 1.10% for400,200,100,50 and 25 μg/ml respectively within IC50 of 98.54 μg/ml. A little effect on viability of normal cells were observed after collagen treatment (72.33±2.41, 76.27±5.12, 83.17±1.31, 94.56±1.51 and 94.13±0.99 %) for 400,200,100,50 and 25 μg/ml respectively within IC50 of 122 μg/ml.