Precision-Cut Lung Slices (PCLS) are uniform, in size and thickness, tissue slices generated from human lungs that can be used for disease modeling, drug discovery and preclinical validation, while retaining the lung's cellular complexity and tissue architecture. This protocol describes two different methods for the induction of cellular senescence by DNA damage in PCLS, and collection of samples for different analysis and measurements.
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Beaulieu et al. (2024) studied this question.
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