February 21, 2024STAR ProtocolsOpen Access

A protocol for isolation and culturing of mouse primary postmitotic photoreceptors and isolation of extracellular vesicles

View Full Paper

Ask AI

Bookmark

Authors

AKAikaterini A. KalargyrouGuy's Hospital AMAyako MatsuyamaGuy's Hospital ELEmily P. LanningCell and Gene Therapy Catapult

Discussion

Member takes

Implication

Protocol demonstrates serum-free isolation and culturing of postmitotic photoreceptors in mice, highlighting an accessible platform for vesicle analysis.

Key Points

The protocol aims to establish reliable isolation and serum-free culturing of primary mouse photoreceptors, utilizing a minimal chemically defined medium to preserve cell viability.
Planned primary outcomes prioritize extracellular vesicles enrichment alongside nanotube formation, tracking structural integrity across multiple murine strains.
Protocol procedures encompass retina dissection and enzymatic dissociation—followed by photoreceptor enrichment, cell culture, and detailed ultrastructural analysis.
May enable downstream studies of extracellular vesicles and intercellular communication—mouse model; further preclinical work needed across diverse systems.

Cite This Study

Kalargyrou et al. (2024) studied this question.

synapsesocial.com/papers/68e785a2b6db6435876f8094 https://doi.org/10.1016/j.xpro.2024.102875

View Full Paper

Ask AI

Bookmark

Implication

Protocol demonstrates serum-free isolation and culturing of postmitotic photoreceptors in mice, highlighting an accessible platform for vesicle analysis.

A protocol for isolation and culturing of mouse primary postmitotic photoreceptors and isolation of extracellular vesicles

Discussion

Member takes

Implication

Key Points

Cite This Study

A protocol for isolation and culturing of mouse primary postmitotic photoreceptors and isolation of extracellular vesicles

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider