What question did this study set out to answer?

To review available retinal pigment epithelial cell culture protocols, assess their effects on cultured tissue phenotype, and identify optimal applications for studying ocular disease.

January 1, 2016Journal of Tissue EngineeringOpen Access

Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations

View Full Paper

Ask AI

Bookmark

Authors

AFAaron Fronk EVElizabeth VargisUtah State University

Discussion

Member takes

Implication

Methodological review evaluates retinal pigment epithelial cell culture protocols across human and animal models, highlighting optimal approaches for modeling age-related macular degeneration.

Key Points

To review available retinal pigment epithelial cell culture protocols, assess their effects on cultured tissue phenotype, and identify optimal applications for studying ocular disease.
Reviewed literature on in vitro cultivation protocols for retinal pigment epithelial cells established since the 1970s.
Evaluated techniques utilizing both human and animal cell sources derived from primary tissue and immortalized cell lines.
Identified marked qualitative differences across protocols in how closely the resulting cultured tissue mirrors natural retinal pigment epithelium in vivo.
Categorized protocol-specific outcomes and provided comparative recommendations to guide experimental models of age-related macular degeneration.

Cite This Study

Fronk et al. (2016) studied this question.

synapsesocial.com/papers/69da065a0d540cafc58381aa https://doi.org/10.1177/2041731416650838

View Full Paper

Ask AI

Bookmark

Implication

Methodological review evaluates retinal pigment epithelial cell culture protocols across human and animal models, highlighting optimal approaches for modeling age-related macular degeneration.

Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations

Discussion

Member takes

Implication

Key Points

Cite This Study

Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider