Antibacterial activity of electrospun nanofibers polyvinylpyrrolidone/cellulose acetate matrix loaded by Ageratum conyzoides L. weed

Evaluating Ageratum conyzoides (bandotan) weed potential, as a natural antibacterial agent that can be used in the biomedical fields, is the objective of this study. Bandotan leaves extract (BLE) loaded PVP/CA nanofibers were fabricated using an electrospinning technique. The resulting PVP/CA/BLE nanofibers exhibit a pure fibers morphology, with average fibers diameter range of 145–346 nm. Fourier transform infrared analysis demonstrates similarities in the spectra of PVP/CA/BLE nanofibers and pure BLE, confirming the effective loading of BLE into the nanofiber matrix. The nanofibers composed of PVP/CA/BLE exhibit significant antibacterial efficacy against both Staphylococcus aureus and Pseudomonas aeruginosa, ranging from 5 to 71 (×10−2 %mg−1), and a high swelling degree of 786%. PVP/CA/BLE nanofibers also exhibit two extract release profiles, burst and sustained release, with a cumulative release of 18–39%. These characteristics make PVP/CA/BLE nanofibers suitable for use as wound dressings, as they can inhibit bacterial growth, absorb wound exudate, and load drug molecules.