Anti Urolithiatic Activity of Dolichos biflorus by Using Wistar Albino Rats in Ethylene Glycol Induction Method

Objective: Dolichos biflorus has been used in the Indian indigenous system of medicine, Ayurveda, for the treatment of various health ailments including renal calculi. The present study was undertaken to investigate the Anti-urolithic activity of ethanolic extract of Dolichos biflorus in Wistar Albino Rats. Method: Dolichos biflorus extract was prepared by using 98% ethanol by maceration method and phytochemical analysis was performed. The samples were examined for antiurolithiatic action by determining phosphate, calcium, and oxalate levels in urine; Urea, uric acid, and creatinine in serum with the Automatic Laboratory Chemistry Research System. The histopathology of the kidney was analyzed. Result: Dolichos biflorus extract at a dosage of 200mg/kg, 400mg/kg in Wistar Albino Rats Significantly, decreased the level of phosphate, calcium as well as oxalate in urine; decreased the serum level of urea nitrogen and creatinine, and uric acid. Comparing the observed outcomes at various dosages to the control groups revealed significant differences. The tubules exhibit normal morphology and have recovered to some extent. These results revealed that the seed extract of Dolichos biflorus decreased calculi levels.