Improved PCR method simultaneously detects FV Leiden and F2 G20210A in thromboembolic diseases, highlighting contamination control.
Factor V (FV) Leiden and F2 G20210A are inherited genetic risk factors that are in the first line of laboratory tests for thromboembolic diseases. Their detection relies on PCR assays that are subject to contamination as well as pipetting error when manually performed and require individual assays for each gene. In this article, we report an im-proved PCR and restriction endonuclease assay for the simultaneous detection of the FV Leiden and F2 G20210A variants, based on multiplex amplification with fluores-cent primers, digestion control, identity monitoring, and contamination tracking.
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Mazancourt et al. (2025) studied this question.
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