October 10, 2025Veterinary PathologyOpen Access

Non-toxic acid-free glyoxal fixative for veterinary gross specimen preservation, histopathology, immunohistochemistry, and molecular analysis

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This study compares glyoxal and formaldehyde in preserving histopathological features, suggesting the former may enhance safety and outcomes.

Glyoxal fixation preserves gross features and cellular ultrastructure better, offering a safer alternative to formaldehyde.
Histology and molecular analysis indicate that glyoxal-fixed samples yield comparable results to those fixed with formaldehyde, despite some differences.
Histochemical techniques such as IHC are effective with glyoxal, with minor protocol adjustments needed for optimal results.
DNA and RNA yields are higher in glyoxal-fixed tissues, enhancing gene amplification efficiency, which can be crucial for further molecular studies.

Zappulli et al. (2025) studied this question.

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This study compares glyoxal and formaldehyde in preserving histopathological features, suggesting the former may enhance safety and outcomes.