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The research aims to evaluate lactic acid as a formaldehyde-free alternative for fixing murine brain tissues.

May 13, 2026Scientific ReportsOpen Access

Evaluation of lactic acid as a novel fixative for histological and neuroanatomical applications

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Randomized trial evaluates lactic acid’s effectiveness as a fixative for brain tissues, suggesting a safer alternative to formaldehyde.

The research aims to evaluate lactic acid as a formaldehyde-free alternative for fixing murine brain tissues.
Mice brain tissues were fixed using immersion or transcardial perfusion with varying concentrations of lactic acid.
Histomorphological preservation was assessed through qualitative, semi-quantitative, and quantitative measurements.
Comparisons were made against neutral-buffered formalin (NBF) and phosphate-buffered saline (PBS).
NBF provided the best overall tissue preservation compared to lactic acid and PBS.
High-concentration lactic acid (LA10–LA20) showed moderate preservation, improving structural integrity and cellular morphology.
Transcardial perfusion with LA20 significantly enhanced preservation compared to immersion alone.

Venuto et al. (2026) studied this question.

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