Whole-mount immunolabeling and clearing method reveals cholera toxin-labelled neurons, indicating their pathways.
The whole-mount immunolabeling and clearing method (iDISCO) was used to visualize cholera toxin subunit B (CTB)-labelled lumbar and sacral preganglionic neurons in the spinal cord of the adult rat. Imaging of spinal cord was performed on a light sheet microscope with a 2x and a 12x lens. Choline acetyltransferase immunoreactivity identified preganglionic autonomic neurons and somatic motoneurons within the spinal cord.
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Fuller‐Jackson et al. (2023) studied this question.
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