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October 12, 2025International Journal of Industrial Engineering

Statistical optimization of L-asparaginase production by E. coli K-12 MTCC 1302

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Authors

JIJ IyyappanGBG. Baskar

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Overview

This research optimizes l-asparaginase production in e. coli K-12 using central composite design, suggesting optimal conditions for enzyme synthesis.

Key Points

  • Optimized l-asparaginase production reached 3.08 U/mL in e. coli K-12 MTCC 1302, showing the effectiveness of optimization techniques.
  • Key independent variables such as lactose, yeast extract, and l-asparagine significantly impacted enzyme production.
  • The plackett-burman experimental design was employed to screen five independent variables linked to fermentation conditions.
  • The central composite design further refined the optimal conditions for l-asparaginase production, allowing for enhanced output.

Cite This Study

Iyyappan et al. (2017) studied this question.

synapsesocial.com/papers/68eb6f7ceea689731b3004cfhttps://doi.org/10.65000/xc397z32
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Improved Extracellular Level of L-Asparaginase in Shaken Cultures of Escherichia coli ATCC 11,303 Using a Statistical Design Approach2026
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  3. 3Optimization of RNA Structure Enhances Biosynthesis of L-Asparaginase from E. coli During Gene Overexpression2025
  4. 4Production of highly cytotoxic and low immunogenic L-asparaginase from Stenotrophomonas maltophilia EMCC22972024 · 2 citations
  5. 5Optimization Studies of Anticancer Enzyme -asparaginase from Actinomycetes2024