Optimization of RNA Structure Enhances Biosynthesis of L-Asparaginase from E. coli During Gene Overexpression

January 1, 2025Прикладная биохимия и микробиология / Applied Biochemistry and Microbiology

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Key Points

To develop an efficient expression system for recombinant L-asparaginase by optimizing RNA structure.
Synthetic gene design for mRNA stability.
Use of E. coli BL21[DE3] strain for protein expression.
Shake-flask cultures for protein production evaluation.
Codon optimization to enhance translation efficiency.
Achieved 291 ± 9 mg/L of active L-asparaginase after 3 hours of induction.
Increased production was 50% higher than the control strain.
Improved mRNA secondary structure stability from –70 to –47 kcal/mol.
Purified enzyme met pharmacopeial standards and displayed high specific activity over 250 IU/mg.

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This research optimizes RNA to improve L-asparaginase production in E. coli, suggesting increased yield in gene overexpression contexts.

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Shaifutdinov et al. (2025) studied this question.

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