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This is a Literature Review study.

2,3-diphosphoglycerate: The Forgotten Metabolic Regulator of Oxygen Affinity

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This review explores how 2,3-DPG affects hemoglobin's oxygen affinity in erythrocytes, highlighting significant gaps in current understanding.

Increased levels of 2,3-DPG shift the oxygen dissociation curve rightward, reducing hemoglobin's oxygen affinity.
Research indicates that dietary phosphorus affects 2,3-DPG levels, which can have implications for conditions like anemia.
Assessing 2,3-DPG in erythrocytes is challenging due to its instability under improper storage conditions.
The impact of physiological factors on 2,3-DPG levels remains inadequately researched, especially in early life.

Jaafar et al. (2025) studied this question.

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This review explores how 2,3-DPG affects hemoglobin's oxygen affinity in erythrocytes, highlighting significant gaps in current understanding.