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October 15, 2025Frontiers in Plant ScienceOpen Access

Re-calibration of flow cytometry standards for plant genome size estimation

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Authors

ASAbhishek SoniRHRobert J Henry

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Overview

Study recalibrates genome size estimates in plants using haplotype assemblies, suggesting improved accuracy.

Key Points

  • Accurate genome size estimation improved by recalibrating with haplotype-resolved assemblies, reducing discrepancies.
  • Using a gapless rice genome assembly as a standard revealed overestimations up to 30% in previous metrics for Pisum sativum.
  • The methodology involved utilizing phased genomes to enhance previous consensus estimates for plant genome sizes.
  • These findings highlight the importance of haplotype-specific data in achieving more reliable estimates for plant genome sizes.

Cite This Study

Soni et al. (2025) studied this question.

synapsesocial.com/papers/68efbd16d61273c8652d8274https://doi.org/10.3389/fpls.2025.1548766
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