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April 17, 2024Journal of the American Society for Horticultural ScienceOpen Access

Flow Cytometry for Estimating Plant Genome Size: Revisiting Assumptions, Sources of Variation, Reference Standards, and Best Practices

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Authors

JNJohn NixTRThomas G. RanneyNLNathan P. Lynch

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Overview

Experimental evaluation reveals up to 57.6% technical variation in plant genome size estimation, highlighting that absolute values are rough approximations dependent on methods.

Key Points

  • Plant genome size estimates by flow cytometry exhibit up to 57.6% cumulative variation across diverse buffers, tissue types, and reference standards.
  • Experimental reassessment of plant standards against human leukocytes revealed fluorochrome discrepancies reaching 32.9% alongside buffer-induced shifts of 18.1%.
  • Highlights that flow cytometry provides precise relative genome size and ploidy measurements, while absolute genome size values remain rough approximations.

Cite This Study

Nix et al. (2024) studied this question.

synapsesocial.com/papers/68e6ead2b6db643587666333https://doi.org/10.21273/jashs05376-24
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Also Consider

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