November 30, 2025Grassland ResearchOpen Access

Flow cytometric DNA ploidy estimation of perennial grasses from the seed collection of Georgikon, Hungary

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Discussion

Analysis reveals genome size variance in perennial grasses, suggesting improvements for plant breeding and grassland studies.

Genome size estimates ranged from 4.31 to 22.69 pg DNA per nucleus, with over fivefold variability observed.
Estimated ploidy levels matched existing data for known accessions, supporting methods for unknown status.
Flow cytometric analysis employed propidium iodide-labeled nuclei across 16 accessions of 13 perennial grass species.
Expanded database may enable rapid identification of DNA ploidy for taxa with unknown cytogenetic status.

Lepossa et al. (2025) studied this question.

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