Evaluation Of Propolis Extract As A Natural Antimicrobial Agent Against Escherichia Coli

Propolis extract's antibacterial efficacy against bacterial isolates taken from enteritis patients was assessed in this study, with an emphasis on multidrug-resistant (MDR) Escherichia coli. The Vitek-2 compact system, morphological, biochemical, and culture techniques were used to identify Gram-positive and Gram-negative bacteria from a total of 30 clinical samples. E. Coli accounted for 30% of all Gram-negative pathogens, with Klebssiella pneumonia and Proteus mirabilis following closely after. High rates of resistance to various widely used antibiotics, such as erythromycin, doxycycline, colistin, and piperacillin, were found in E. coli isolates by antibiotic susceptibility testing; nevertheless, ciprofloxacin and cefotaxime continued to exhibit notable action. Using the disc diffusion method, propolis extract was produced in varying quantities and tested against isolates of MDR E. coli. The findings showed that propolis had a definite dose-dependent inhibitory effect, with certain isolates exhibiting inhibition zones that were on par with or greater than those of ciprofloxacin. At 100 mg/ml, the greatest action was seen, resulting in inhibition diameters of up to 26 mm. These results suggest that propolis has promising antibacterial properties and could be used as a supplemental or natural medicinal treatment to combat enteritis-causing bacteria that are resistant to antibiotics.