Selection of Native Isolates of Trichoderma Spp on Different Natural Substrates for Mass Multiplication

The need to reduce the use of synthetic fungicides for crop pest control makes it necessary to evaluate and develop technologies that allow products to be obtained from microorganisms in an easy, economical, and effective way for their mass application in cultivated areas. This study was conducted to identify prominent strains of the genus Trichoderma and to determine suitable organic substrates for use in mass propagation. The yield and viability variables were established in a completely randomized experimental design (CRD). Native strains of Trichoderma spp T58, T38, T36, T52, T49, and FRAIII were evaluated, as were organic substrates such as sorghum, corn, 96% whole grain rice, 80% whole grain rice, 70% whole grain rice, and 50% whole grain rice. The mass production method was semi-industrial two-phase. The data were organized using Microsoft Excel software and then processed with the Infostat 2020 statistical package. The normality of the data and the homogeneity of the variances were analyzed. The comparison of means was performed using the Tukey test (p < 0.005). The organic substrates with the best spore yields were 96% whole grain rice, 80% whole grain rice, and 70% whole grain rice. The substrate with the best spore viability was the organic substrate based on 80% whole grain rice. The strain with the best yield was strain T49, and the strains with the best viability percentages were strains FRAIII, T36, and T38.