Methodological Features of Verification of Biological Models of Pathological Conditions

This work is aimed at improving the quality of biological modeling of pathological conditions in laboratory animals used for the purposes of developing new medicines. The methodological features of the development, verification, and standardization of new biomodels of pathological conditions for relevant and alternative biomodeling, ensuring their compliance with the requirements of evidence-based medicine, are considered. The development of new models should be based on pathogenetically inducing pathological effects similar to human and animal biomodels. The optimality of the model should be studied at several levels of inducing effects, and the research methods should allow quantifying the severity of the modeled pathological condition. It is shown that the verification of new biological models can be implemented in the process of their pharmacological validation using at least two drugs with a known mechanism of action, exhibiting both activating and suppressing pathogenetically determined effects on the severity of the studied pathological condition. The criterion for a sufficient degree of standardization of the model can be high indicators of intra-laboratory and precision convergence of modeling results.