REFLEX, a Novel Immune Profiling Assay, Combining TCR Repertoire and Multiome at Massively Scalable Single-cell Resolution to Catapult Exploration of T-cell Derived Immunity

Single-cell profiling of TCR repertoire is crucial to understanding heterogeneous responses to antigen. Existing technologies struggle to combine paired TCRαβ sequence with multiomic profiling at sufficient scale and cost. We present ″REFLEX″, a novel single-cell method enabling highly scalable, cost efficient, multi-modal profiling with TCR repertoire. We demonstrate the power of REFLEX by profiling evolving cell states and TCR clonotypes for >2 million cells from virus-specific T-cell expansions with/without metabolic perturbation.