What question did this study set out to answer?

To present TIRTL-seq, a new method for sequencing paired TCRs from live cells rapidly and affordably.

November 1, 2025The Journal of ImmunologyOpen Access

TIRTL-seq: Deep, quantitative, and affordable paired TCR repertoire sequencing 3040

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To present TIRTL-seq, a new method for sequencing paired TCRs from live cells rapidly and affordably.
Developed a novel sequencing approach called TIRTL-seq for TCR repertoire analysis.
Used automated protocols for liquid handling in 384-well plates.
Benchmark against existing TCR sequencing technologies on longitudinal samples.
TIRTL-seq can identify up to 1 million unique paired TCR clonotypes affordably.
Demonstrated precise inference of clone frequencies and TCR chain pairings.
Showed differential dynamics in SARS-CoV-2 specific and EBV-specific T cell responses.

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Novel TIRTL-seq identifies dynamics of expanding clonotypes in adaptive immunity, suggesting potential therapeutic insights.

Pogorelyy et al. (2025) studied this question.

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