Isolation and Identification of Staphylococcus Aureus from Raw Chicken Meat, Obtained from Retail Slaughtered Houses in Karachi and Its Susceptibility against Commonly Used Antibiotics

Microbial pollution of poultry carcasses is a natural final result of different operation necessary to produce retailed products from living birds. Most of bacterial contaminant are non pathogenic; however, poultry are known to haven a large figure of bacteria that are pathogenic to human being. Staph aureus is an opportunistic pathogen which has emerged as a significant cause of nosocomial infection. The present study revealed the presence of several microbial populations such as Enterococcus spp, S. aureus and coagulase negative staphylococci and they were found to be resistant towards several antibiotic. In this study a total of 20 samples of chicken heart, liver and muscles meat were collected and processed for the isolation of S. aureus, which was 30%, 25% and 35% respectively. Isolates were then preceded for antibiotic susceptibleness towards commonly used drugs. All the isolates were found to be resistant towards Amoxycillin/Clavulanic acid (n = 15, 75%), Azithromycin (n =14, 70%), Cefoxitin (n= 3, 15%), Ceftazidime (n= 03, 15%), Chloramphenicol (n= 06, 30%), Ciprofloxacin (n = 02, 10%), Clarithromycin (n= 01, 05%). Levofloxacin (n= 03, 15%), Methicillin (n= 12, 60%) and Ofloxacin (n = 11, 55%). These results suggest that S. aureus is highly prevalent in retail meat in Different field of Karachi and many isolates are resistant to commonly used drug.