Recent Advances in Electrochemiluminescence Microscopy Combined With Multiple Regulations

This article reviews the latest developments in electrochemiluminescence microscopy (ECLM) technology, which combines electrochemical input and optical output, and features low background noise, high controllability, as well as high spatial resolution. Through various adjustment strategies such as temperature regulation, ultrasonic enhancement, electro field regulation, and optical regulation, researchers have achieved precise control of ECL signals, thus improving the measurement sensitivity of ECLM. These technologies provide new strategies and tools for biological imaging, clinical testing, and nanomaterial analysis. For example, efficient ECL emission and direct modulation of ECL intensity at low potentials were achieved through a metal‐insulator‐semiconductor structure or an external magnetic field. In addition, the application of aggregation‐induced luminescence effect demonstrates the potential of ECLM in improving the specificity of sensors and solving aggregation quenching problems. Despite the challenges, continuous innovation of ECLM technology is expected to bring wider applications in the biomedical field.