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November 14, 2025Frontiers in Bioengineering and BiotechnologyOpen Access

Efficient production of functional cholera toxin B subunit using geminiviral replicons in Nicotiana benthamiana

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Authors

NKNan Sun KimJLJihyea LeeJLJuho Lee

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Overview

Study demonstrates enhanced cholera toxin B subunit expression in Nicotiana benthamiana, suggesting potential as an adjuvant in mucosal vaccines.

Key Points

  • The aim is to express the cholera toxin B subunit at high levels using geminiviral systems in plants.
  • Utilized geminiviral replicons for transient expression in Nicotiana benthamiana.
  • Cloned plant-optimized CTB gene into geminivirus vectors and co-infiltrated into plant leaves.
  • Conducted immunoblot analysis to verify synthesis and assembly of CTB into pentamers.
  • Achieved approximately 2.5 mg/g fresh weight of CTB protein on day 5.
  • Demonstrated that CTB forms an active pentamer.
  • Showed CTB has a strong affinity for GM1-ganglioside.

Cite This Study

Kim et al. (2025) studied this question.

synapsesocial.com/papers/692519a7c0ce034ddc354066https://doi.org/10.3389/fbioe.2025.1693569
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  5. 5Development of Modular Geminivirus‐Based Vectors for High Cargo Expression and Gene Targeting in Plants2025