Simulation of the glycolytic metabolites concentration profile in mammalian resting skeletal muscles

For the first time, a mathematical model of glycolysis in mammalian skeletal muscles is presented, in which stationary concentrations of glycolysis metabolites are in good agreement with experimental data obtained in resting muscles. The correspondence between the model and experimental values of metabolite concentrations was achieved due to enhancing the inhibitory effect of ATP on pyruvate kinase and significantly reducing the ratio of [NAD]/[NADH] concentrations in the cytoplasm of skeletal muscles. At the same time, in order for glycolysis to provide the rate of ATP production necessary for activation of muscle load, an activation of muscle pyruvate kinase by fructose-1,6-diphosphate was included in the model.