Detection method of Aristolochic acid I based on magnetic carrier Fe ₃ O ₄ and gold nanoclusters

Aristolochic acid I (AA-I) has been proven to be toxic, and there has been a global research frenzy to test it. To simplify the detection steps and enhance detection efficiency, this study proposes a detection method utilizing magnetic carriers such as Fe 3 O 4 and gold nanoclusters. To enrich the AA-I in the sample, this study first designs a magnetic carrier based on ferric oxide, and then synthesizes a porcine serum albumin gold nanocluster fluorescent needle for the detection of AA substances. The magnetic molecularly imprinted polymer of AA-I could be completely separated from ultrapure water within 8 s. The maximum imprinting efficiency factor value was obtained when the mass ratio of “template molecule: crosslinking agent: functional monomer” was 1:20:4. The methyl acrylic acid was used as the functional monomer, and acetonitrile methanol was used as the solvents. As the temperature increased from 30 to 80°C, the fluorescence quenching intensity briefly increased and then gradually decreased from about 0.4 to nearly 0.1. The most suitable temperature was approximately 38°C. The proposed method can effectively collect and detect AA-I, has practicality, and can provide ideas for the development of AA-I detection methods.