December 2, 2025Nature CommunicationsOpen Access

Divergent evolution of fungal P450 monooxygenase unlocks simultaneous access to C12β and C15α oxyfunctionalization of steroids

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Divergent evolution enhances hydroxylation of progesterone in engineered strains, indicating scalable steroid synthesis potential.

C15α-hydroxylation exhibits 99.6% selectivity for progesterone, demonstrating effective optimization.
Molecular dynamics simulations reveal key mutations reshaping the binding pocket's polarity.
High-density fermentation with Pichia pastoris yields up to 14.1 g/L of hydroxylated steroids.
Result indicates broad applicability for producing complex bioactive molecules like drospirenone.

Chenghua Gao (2025) studied this question.

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December 2, 2025Nature CommunicationsOpen Access

View Full Paper

Ask AI

Bookmark

Authors

Divergent evolution enhances hydroxylation of progesterone in engineered strains, indicating scalable steroid synthesis potential.

C15α-hydroxylation exhibits 99.6% selectivity for progesterone, demonstrating effective optimization.
Molecular dynamics simulations reveal key mutations reshaping the binding pocket's polarity.
High-density fermentation with Pichia pastoris yields up to 14.1 g/L of hydroxylated steroids.
Result indicates broad applicability for producing complex bioactive molecules like drospirenone.

Chenghua Gao (2025) studied this question.

View Full Paper

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Bookmark