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February 21, 2026Synthetic and Systems BiotechnologyOpen Access

P450 engineering via structure-guided rational design achieves high C21-selectivity and bioconversion in steroid biosynthesis

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Authors

JYJian YangRLRong LiQGQilin Gao

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Overview

Engineering cyp154c5 improves C21-selectivity and bioconversion in steroid biosynthesis, indicating potential for efficient steroid production.

Key Points

  • This research aims to enhance C21-selectivity and catalytic efficiency in steroid biosynthesis through engineered P450 enzymes.
  • Engineered CYP154C5 using structure-guided design and site-directed mutagenesis.
  • Created variants M6a and M6a-RhFRED L3 with improved catalytic activity.
  • Performed molecular docking and dynamics simulations to study substrate interactions.
  • M6a variant achieved 98% selectivity for C21-hydroxylation of progesterone.
  • M6a-RhFRED L3 showed a 1.43-fold improvement in catalytic activity compared to M6a-RhFRED.
  • M6a-RhFRED L3 also catalyzed three steroid analogs with 98% selectivity and over 60% conversion rates.

Cite This Study

Yang et al. (2026) studied this question.

synapsesocial.com/papers/69994ba9873532290d01fcb9https://doi.org/10.1016/j.synbio.2026.01.034
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Also Consider

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