What question did this study set out to answer?

The aim is to improve the bioconversion of steroid drugs using a high-performance enzyme isolated from fungi.

Multidimensional Engineering Delivers a High-Performance C11α-Steroid Hydroxylase for Practical Application

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Multidimensional engineering demonstrates improved steroid bioconversion, highlighting sustainable techniques in fungi.

The aim is to improve the bioconversion of steroid drugs using a high-performance enzyme isolated from fungi.
Isolation of CYP68N3_ma from Metarhizium anisopliae.
Expression of CYP68N3_ma in Saccharomyces cerevisiae for steroid conversion.
Iterative saturation mutagenesis to create the mutant N3M3 for enhanced activity.
Molecular dynamics and near-attack analysis to study enzyme-substrate interactions.
Expression in Komagataella phaffii to further improve conversion rates.
Achieved a concentration of 2.21 mM of 11α,17α-dihydroxyprogesterone with triple mutant N3M3.
Overexpression of heme synthesis and transport proteins led to 24.8 g/L in high-cell density bioreactor.
Demonstrated a 13.8-fold increase in steroid conversion with the engineered mutant.

Wei et al. (2026) studied this question.

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