Identification and Quantitation of 14C-Labeled Catechol Metabolites in Rat Plasma After Intranasal Instillation of Smoldering Eucalyptus Wood Smoke Extract

The increasing frequency, duration, and intensity of wildfires over the past decade have raised significant concerns about widespread exposure to wildfire smoke. Inhalation of wildfire smoke poses a substantial risk to human health, with epidemiological studies linking exposure to cardiovascular, respiratory, and neurological dysfunction. Wildfire smoke contains hundreds of chemical compounds across diverse classes, with concentrations varying by fuel type and combustion conditions. Phenolic compounds are prominent constituents of wood smoke, and catechol is especially abundant under smoldering conditions that produce dense smoke. In this study, 14C-labeled catechol was spiked into smoldering eucalyptus wood smoke extract (WSE) and administered to rats via intranasal instillation. Plasma was collected at 5 min and 2 h post-exposure. Samples were analyzed using parallel accelerator and molecular mass spectrometry (PAMMS). Major catechol-derived metabolites identified included benzene oxide, catechol-cysteine conjugate, and catechol-glutamine conjugate; the parent compound was not detected. These results indicate that inhaled catechol in wood smoke is quickly metabolized upon entry into circulation. PAMMS enabled both identification and relative quantification of circulating catechol metabolites, demonstrating feasibility for biomarker discovery and exposure assessment.