Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
December 8, 2025Biochemistry (Moscow)Open Access

Reporter System for Detection of G-Quadruplexes in the Human Telomerase Reverse Transcriptase Gene Promoter Region

View Full Paper
Ask AI
Bookmark
Share

Authors

ЕКЕ. А. Кубарева

Discussion

Loading...

Member takes

Overview

The study demonstrates G-Quadruplexes formation in the hTERT promoter, suggesting new antitumor therapies using fluorescent proteins.

Key Points

  • This research aims to explore G-Quadruplexes in the hTERT gene promoter and their potential impact on tumor cell expression.
  • Studied the G-rich region of the hTERT promoter for G-Quadruplexes formation.
  • Developed plasmid constructs containing fluorescent protein genes to visualize G-Quadruplexes.
  • Conducted DNA polymerase stop assay to confirm G-Quadruplex formation.
  • G-Quadruplexes were successfully formed in the hTERT promoter region constructs.
  • Stabilization of G4 structure may reduce hTERT expression in tumor cells.
  • Low-molecular weight ligands effectively interacted with the G4 structure at specific concentrations.

Cite This Study

Е. А. Кубарева (2025) studied this question.

synapsesocial.com/papers/693624ce4fa91c937236cd69https://doi.org/10.1134/s0006297925601753
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1THE REPORTER SYSTEM FOR THE DETECTION OF G-QUADRUPLEXES IN THE PROMOTER REGION OF THE HUMAN TELOMERASE REVERSE TRANSCRIPTASE GENE2025
  2. 2Targeting hTERT Promoter G-Quadruplex DNA Structures with Small-Molecule Ligand to Downregulate hTERT Expression for Triple-Negative Breast Cancer Therapy2024 · 33 citations
  3. 3Modulation of dynamic DNA G-quadruplex structures in the hTERT promoter region by ligands2024 · 12 citations
  4. 4Studying the Pathogenic Effect of New Nucleotide Changes in the Promoter Region of the TERT Gene in Patients with Glioblastoma Brain Tumor2024 · 1 citations
  5. 5Stable G quadruplex formed in promoter region of oncogene RET in physiological solution conditions2024 · 3 citations