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December 8, 2025BloodOpen Access

Spatial transcriptomics identified dynamic and spatial-resolved niche cell and signal architecture for hematopoietic stem cell specification

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Authors

XMXinjian MaoXHXi HeZYZhe Yang

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Overview

Spatial transcriptomics identifies notch signaling and pluripotent stem cells essential for hematopoietic stem cell specification.

Key Points

  • This research aims to elucidate the cellular composition and signaling landscape within the hematopoietic stem cell niche.
  • Utilized single-cell RNA sequencing and spatial transcriptomics (Curio-seq)
  • Analyzed AGM region at embryonic days E10.5, E11.5, and E12.5
  • Examined cellular distributions between dorsal and ventral aortae
  • Verified findings with MERFISH imaging-based spatial transcriptomics
  • Cultured reaggregated AGM cells to assess HSC maturation.
  • Identified distinct cellular distributions in the aorta with enriched N-cad+ cells on the dorsal side
  • Mapped notch signaling and interactions between niche cells and pre-HSCs
  • Depletion of N-cad+ MSCs reduced functional hematopoietic stem cells
  • Jag1 supplementation improved HSC specification impaired by N-cad+ MSC depletion.

Cite This Study

Mao et al. (2025) studied this question.

synapsesocial.com/papers/693624d44fa91c937236d077https://doi.org/10.1182/blood-2025-3151
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Also Consider

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  1. 1A multistep computational approach reveals a neuro-mesenchymal cell population in the embryonic hematopoietic stem cell niche2024 · 12 citations
  2. 2Restraint of TGF-β signaling by SMAD7 is necessary for the maturation of hematopoietic stem cells in the mouse embryo2025
  3. 3A subset of megakaryocytes regulates development of hematopoietic stem cell precursors2024 · 10 citations
  4. 4Spatial transcriptomic interrogation of the murine bone marrow signaling landscape2023 · 54 citations
  5. 5Single-cell atlas of major haematopoietic tissues sheds light on blood cell formation from embryonic endothelium2019