What question did this study set out to answer?

The aim is to quantify mutation-specific clonal fitness and lineage bias in MPNs to improve event-free survival.

December 8, 2025BloodOpen Access

Functional annotation of MPN clones reveals mutation-specific fitness and fate bias

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Authors

FTFranco Castillo TokumoriCornell University

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Implication

Single-cell pipeline reveals mutation-specific fitness and lineage tracking in MPN clones, indicating potential therapeutic strategies to address thrombosis and leukemic transformation.

Key Points

The aim is to quantify mutation-specific clonal fitness and lineage bias in MPNs to improve event-free survival.
Developed a single-cell pipeline integrating immunophenotype and genotype to assess clonal fitness in MPNs.
Utilized MissionBio Tapestri platform for automated single-cell genotyping of primary patient samples.
Analyzed lineage tracking and fitness of hematopoietic clones using machine learning models on over 80,000 cells.
JAK2V617F clones showed different fitness based on zygosity and co-occurring mutations.
Monoallelic and biallelic JAK2V617F clones favored myeloid over lymphoid differentiation, with varying fitness levels.
Fitness tracking revealed distinct differentiation trajectories among JAK2V617F clones, impacting outcomes related to thrombosis and leukemic transformation.

Cite This Study

Franco Castillo Tokumori (2025) studied this question.

synapsesocial.com/papers/693624d74fa91c937236d12b https://doi.org/10.1182/blood-2025-3766

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Implication

Single-cell pipeline reveals mutation-specific fitness and lineage tracking in MPN clones, indicating potential therapeutic strategies to address thrombosis and leukemic transformation.

Functional annotation of MPN clones reveals mutation-specific fitness and fate bias

Discussion

Member takes

Implication

Key Points

Cite This Study

Functional annotation of MPN clones reveals mutation-specific fitness and fate bias

Discussion

Member takes

Implication

Key Points

Cite This Study

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