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December 8, 2025BloodOpen Access

Non-genotoxic conditioning to increase donor chimerism levels in a mismatched murine transplant model for sickle cell disease

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Authors

AIAyotimofe IdowuMAMohamed AhmedCFCourtney D. Fitzhugh

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Overview

Non-myeloablative conditioning improves donor chimerism in sickle cell disease, indicating potential for better outcomes.

Key Points

  • The study aims to explore a non-genotoxic conditioning approach to increase donor chimerism in sickle cell disease through a murine transplant model.
  • Utilized a mismatched murine HCT model for sickle cell disease.
  • Evaluated conditioning agents CD45-sap and CD117-sap against traditional total body irradiation and busulfan.
  • Applied post-transplant cyclophosphamide and sirolimus in the conditioning regimen.
  • Performed long-term follow-up to assess chimerism levels and hemoglobin restoration.
  • CD117-sap conditioned mice showed significantly higher donor myeloid chimerism levels compared to total body irradiation.
  • Blood transfusions prior to conditioning restored hemoglobin levels in sickle cell model mice.
  • Bone marrow analysis confirmed increased frequencies of donor-derived hematopoietic stem cells in CD117-sap conditioned recipients.

Cite This Study

Idowu et al. (2025) studied this question.

synapsesocial.com/papers/69362f484fa91c937236d68ehttps://doi.org/10.1182/blood-2025-5858
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Defining therapeutic chimerism thresholds for comprehensive sickle cell disease correction in the townes mouse model2025
  2. 2Refined design of a CD117 antibody-drug conjugate safely and effectively conditions non-human primates for autologous transplant-based gene therapy2025
  3. 3Computational dissection of immune reconstitution across diverse conditioning regimens in adults with sickle cell disease undergoing allogeneic stem cell transplantation2025
  4. 4Azathioprine/hydroxyurea preconditioning prior to nonmyeloablative matched sibling donor hematopoietic stem cell transplantation in adults with sickle cell disease: A prospective observational cohort study2024 · 10 citations
  5. 5Research Highlights2026